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Coding

Part:BBa_J52224:Design

Designed by: Jelka Pohar   Group: iGEM06_Slovenia   (2006-10-09)


dnMyD88-linker-GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1177
    Illegal PstI site found at 1191
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 1177
    Illegal PstI site found at 1191
    Illegal NotI site found at 7
    Illegal NotI site found at 1184
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1177
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1177
    Illegal PstI site found at 1191
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In dnMyD88 there is mutation, bacause original sequence has PstI restriction site.


Source

dnMyD88 - GFP - more information later

References